KMID : 0880220110490020265
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Journal of Microbiology 2011 Volume.49 No. 2 p.265 ~ p.273
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Transcriptional control of genes involved in yeast phospholipid biosynthesis
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Roshini Wimalarathna
Chen-Han Tsai Chang-Hui Shen
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Abstract
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Phospholipid biosynthetic genes encode enzymes responsible for phospholipid biosynthesis. They are coordin-ately regulated by the availability of phospholipid precursors through the inositol-sensitive upstream activating sequence (UASINO). However, not all phospholipid genes are UASINO-containing genes and not all UASINO-containing genes have the same response to the phospholipid precursors. Therefore, the transcriptional regulation of phospholipid genes in response to the availability of phospholipid precursors is still unclear. Here, 22 out of 47 phospholipid biosynthetic genes were identified as UASINO-containing genes, including EKI1, EPT1, INM1, IPK2, KCS1, PAH1, and PIK1 which have never been reported before. We also showed, using qRT-PCR technique, that 12 UASINO-containing genes are down-regulated by 100 ¥ìM inositol in the wild type cells and up-regulated by 100 ¥ìM inositol in the ino2¥Ä cells. Therefore, it is possible that these genes are transcriptionally regulated by the UASINO through the negative response of Ino2p to inositol. One other UASINO-containing gene might be regulated by the positive response of Ino2p to 100 ¥ìM inositol. Surprisingly, we found 9 UASINO-containing genes are not dependent on the response of Ino2p to 100 ¥ìM inositol, indicating that they may be regulated by other pathway. Furthermore, we identified 9 and 3 non-UASINO-containing genes that are possibly regulated by the negative and positive response of Ino2p to 100 ¥ìM inositol, respectively. Therefore, these observations provide insight into the understanding of the co-regulated phospholipid biosynthetic genes expression.
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KEYWORD
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phospholipid biosynthesis, gene expression, UASINO, Ino2p, qRT-PCR
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